Figure 6. Loss of Bhlhb5 Function Results in Loss of SCPN and CSMN Molecular Features in Caudal Motor Cortex.
In situ hybridization on coronal (20 µm thick) and sagittal (30 µm thick) brain sections of P5 wild type and Bhlhb5lacZ/lacZ (nulls) for subcerebral projection neuron markers, (A1–A4) S100A10, (B1–B4) mu-crystallin and (C1–C4) Crim1, reveals markedly reduced expression of these markers in caudal but not rostral motor cortex (wild type n=3; Bhlhb5-nulls n=3). Scale bars: 500 µm.