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Systematic identification of binding determinants in the RseA 169-196 peptide. The His-Trx-RseA 169-196 peptide containing single alanine substitution mutations were purified and tested in vitro for binding to RseB as described in the legend to Fig. 1 and “Experimental Procedures.” Relative binding of various mutant peptides to wild-type RseB is shown [(RseBwt/RseAmut)/(RseBwt/RseAwt) × 100].
