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Allele-specific suppression analysis of RseA and RseB. The His-Trx-RseA 169-196 peptide containing single alanine substitution mutations were purified and tested in vitro for binding to Flag-tagged WT RseB (light gray) [(RseBwt/RseAmut)/(RseBwt/RseAwt) × 100] or Flag-tagged I249A+E250A RseB mutant (dark gray) [(RseBmut/RseAmut)/(RseBwt/RseAwt) ×100].
