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. 2009 Feb 20;284(8):4760–4766. doi: 10.1074/jbc.M805464200

FIGURE 2.

FIGURE 2.

Effects of IKK inhibition on ICAM-1 expression in Grx1 overexpressing rMC-1 cells. rMC-1 cells were cultured in normal glucose (5 mm) medium and pretreated with Bay 11-7085 (Bay) for 30–40 min. Then the cells were infected immediately with m.o.i. of 10 of either Ad-Grx1 or Ad-Empty, cultured for 16–24 h in normal glucose medium, and collected in Nonidet P-40 lysis buffer for immunoblotting of ICAM-1 (1:1000) and actin (1:30,000) (n = 7). Ad-Grx1 increased ICAM-1 expression by 2.6-fold (±0.5), *, p < 0.01. ICAM-1 expression of Ad-Grx1-infected cells treated with 10 μm Bay 11-7085 was statistically increased, but cells treated with 20μm Bay 11-7085 expressed ICAM-1 in similar amounts as control cells (no adenovirus and Ad-Empty m.o.i. of 10). In addition, ICAM-1 of cells infected with Ad-Grx1 and treated with 10 and 20 μm inhibitor was significantly decreased from Ad-Grx1-infected cells with no inhibitor, #, p < 0.01.