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. 2009 Feb 20;284(8):4760–4766. doi: 10.1074/jbc.M805464200

FIGURE 3.

FIGURE 3.

Effects of IKK inhibition on nuclear translocation of NFκB p50 and p65 in Grx1 overexpressing rMC-1 cells. rMC-1 cells were cultured in normal glucose (5 mm) medium, pretreated with Bay 11-7085 (Bay) for 30 min prior to Grx1 overexpression, immediately infected with m.o.i. of 10 of either Ad-Grx1 or Ad-Empty, cultured for two more days in normal glucose medium, and collected. Cells were separated into nuclear fractions, run on a 12% SDS-PAGE, and immunoprobed for p50 (1:1,000), p65 (1:3,000), and the nuclear loading control YY1 (1:1,000). Ad-Grx1 increased p50 in the nucleus by 4-fold (±0.7) (n = 9) (panel a), *, p ≤ 0.002. Cells overexpressing Grx1 treated with 10 and 20 μm Bay 11-7085 were significantly decreased from Grx1-overexpressing cells with no inhibitor (n = 9) (panel a), #, p < 0.02. Ad-Grx1 increased p65 in the nucleus by nearly 2-fold (±0.7) (n = 11) (panel b), *, p ≤ 0.002. Cells overexpressing Grx1 treated with 10 and 20 μm Bay 11-7085 were significantly decreased from Grx1-overexpressing cells with no inhibitor (n = 11) (panel b), #, p < 0.02.