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. 2009 Feb 20;284(8):4760–4766. doi: 10.1074/jbc.M805464200

FIGURE 6.

FIGURE 6.

ICAM-1 expression in rMC-1 cells treated with conditioned medium from Grx1 overexpressing rMC-1 cells. rMC-1 Müller cells were transfected with Ad-Grx or Ad-Empty at m.o.i. of 10 or m.o.i. of 20, and the C.M. was collected from these cells after 24 h. C.M. was then placed on newly cultured rMC-1 Müller cells for 24 h, and the cell lysate was processed for Western blot analysis. C.M. from rMC-1 overexpressing two concentrations of Grx1 (Ad-Grx1 m.o.i. of 10 and m.o.i. of 20) led to an increase of 2.4-fold (±0.6) and 2.9-fold (±0.7) in Grx1, respectively (n = 6), *, p ≤ 0.03. ICAM-1 was increased by 1.7-fold (±0.3) and 2.2-fold (±0.5) in response to conditioned medium from rMC-1 cells overexpressing Grx1 at m.o.i. of 10 or m.o.i. of 20, respectively (n = 6), *, p ≤ 0.04.