DGAT2 is present in microsomes (Micro.) and enriched in
MAM. A, subcellular fractions were isolated from McArdle cells
stably expressing DGAT2. An equal amount of protein (30 μg) from each
fraction was analyzed by immunoblotting with anti-DGAT2, anti-calnexin, and
anti-cytochrome C (Cyt C) antibodies (Mito., mitochondria).
B, in vitro DGAT activity of microsomes and mitochondria plus MAM
(mito./MAM) from livers of DGAT knockout mice fed a high fat diet for
1 week. Data are from one experiment, performed in triplicate, which was
repeated once with similar results. *, p = 0.038
versus mean for microsomes.