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. 2009 Feb 20;284(8):5352–5361. doi: 10.1074/jbc.M805768200

FIGURE 4.

FIGURE 4.

DGAT2 is present in microsomes (Micro.) and enriched in MAM. A, subcellular fractions were isolated from McArdle cells stably expressing DGAT2. An equal amount of protein (30 μg) from each fraction was analyzed by immunoblotting with anti-DGAT2, anti-calnexin, and anti-cytochrome C (Cyt C) antibodies (Mito., mitochondria). B, in vitro DGAT activity of microsomes and mitochondria plus MAM (mito./MAM) from livers of DGAT knockout mice fed a high fat diet for 1 week. Data are from one experiment, performed in triplicate, which was repeated once with similar results. *, p = 0.038 versus mean for microsomes.