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The WD40 repeats of UAF1 are required for the interaction with USP12 and USP46. A, scheme of wild-type UAF1 and deletion mutants thereof. B, Flag-tagged deletion mutants of UAF1 were transiently expressed in 293T cells. The Flag-tagged proteins were immunoprecipitated from cell lysates using anti-Flag antibodies. The immunoprecipitates were analyzed using the indicated antibodies.
