FIGURE 5.

Ligand modulates monomer-dimer equilibrium in favor of dimerization. A–C, gel filtration of Gal-1 at 10 (A), 2.5 (B), and 0.25 μm (C). D, quantification of the percent dimer formation following gel filtration for each indicated concentration in the presence or absence of ligand. E, gel filtration analysis of 0.25 μm Gal-1. Following analysis, monomeric fractions were concentrated to 2.5 μm Gal-1 and reanalyzed in F. G and H, mGal-1 was subjected to gel filtration analysis in either the absence (G) or presence (H) of ligand. D, dimer; M, monomer.