FIGURE 6.
mGal-1 displays impaired capacity to be stabilized by ligand. A, incubation of 40μm Gal-1 in PBS, with or without 2-ME or lactose as indicated followed by determination of the soluble fraction. B, incubation of 40 μm mGal-1 in PBS, with or without 2-ME or lactose as indicated followed by determination of the soluble fraction. C and D, surface plasmon resonance analysis of the binding of Gal-1 (C) or mGal-1 (D) to lactose. E, fluorescent image acquired following SDS-PAGE of BS3 chemically cross-linked Alexa Fluor 488-labeled Gal-1 or mGal-1. D, dimer; M, monomer.