TABLE 5.
Comparison of AAK-NAT domain interactions between R- and T-state structures
|
R-state structure |
T-state structure |
||||
|---|---|---|---|---|---|
| AAK domain | NAT domaina | Distance | AAK domain | NAT domaina | Distanceb |
| Å | Å | ||||
| Ile136 O | Asn394 OD1 | 2.80 | Arg134 NE | Asn323 OD1 | 3.23,2.94 |
| Val138 N | Thr393 O | 2.66 | Pro135 O | Asn323 ND2 | 3.17,3.04 |
| Val138 O | Thr393 OG1 | 2.75 | Arg151 NH2 | Glu322 OE2 | 2.67,2.42 |
| Asp143 OD2 | His428 NE2 | 2.56 | Arg151 NH2 | Glu322 O | 3.25,3.25 |
| Arg151 NH1 | Glu401 OE2 | 3.30 | Ser176 OG | Glu322 OE1 | 3.51,3.45 |
| Arg151 NH2 | Glu401 OE1 | 3.54 | Gly178 N | Glu322 OE1 | 2.83,2.93 |
a
NAT (S) domain from an adjacent subunit in the R- or T-state.
b
The values are for two subunits (subunit A and B) in the asymmetrical unit, respectively.