TABLE 1.
Inhibition of recombinant LeCCD1a and LeNCED1 enzymes using in vitro assays
Enzyme assays, using E. coli cell extracts containing the recombinant CCD, were initially carried out at 100 μM inhibitor concentration; for compounds showing ≥95% inhibition of LeCCD1a at this concentration, IC50 values were also determined. NT, not tested. Chemical structures of hydroxamic acid inhibitors are shown below, with X and Y given in the table. The structure of abamine is given in Fig. 3.
a Mean and range of two independent experiments.