FIGURE 8.

MASM cell confluency and its differential effect on tunicamycin and poly(I,C)-induced hyaluronan masking. A shows the FACE analysis of hyaluronan for MASM cells plated at pre-confluency (white bars), confluency (gray bars), and post-confluency (black bars) (n = 4). The data for the nontreated cells and the tunicamycin-treated cells were multiplied ×3 for better visualization. B shows the number of leukocytes bound via hyaluronan to the MASM cells for this same experiment (n = 3). C contrasts hyaluronan measurement from MASM cells using a fluorescent-tagged HABP (which measures the amount of available hyaluronan surface area) with FACE analysis (which gives the total hyaluronan mass) presenting the data as fold increase above untreated. Error bars represent standard deviation.