TABLE 1.
Morphometric analysis of structural changes induced by in vivo administered PAF in the murine lung vascular bed
The vascular segments were identified, on micrographs at ×42,500 final magnification, based on their luminal diameters, 30 mm for arteries, between 10 and 20 mm for arterioles, <9 mm for capillaries, between 10 and 30 mm for venules, and 40 mm for veins. n = number of micrographs on which the vascular segment was identified. *, p < 0.001; ‡, p < 0.01. BM, basement membrane.
|
Vascular segment
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Aggregated area
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Open junctions
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Fenestrations
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BM thickness
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Large vesicles (>200 nm)
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control | PAF | Control | PAF | Control | PAF | Control | PAF | Control | PAF | |||||
| μm2 | % of total | No./μm length | μm | |||||||||||
| Artery (n = 98) | 47 ± 5 | 55 ± 8 | 0 | 0 | 0 | 0 | 0.32 ± 0.05 | 0.34 ± 0.08 | 0 | 0 | ||||
| Arteriole (n = 122) | 86 ± 1 | 79 ± 4 | 0 | <0.5 | 0 | 1.5 ± 0.3* | 0.33 ± 0.07 | 0.32 ± 0.09 | 0 | 11 ± 2 | ||||
| Capillary (n = 388) | 568 ± 29 | 487 ± 39 | 0 | 33 ± 7* | 0 | 5.4 ± 0.6* | 0.29 ± 0.07 | 0.51 ± 0.06‡ | 18 ± 3 | 227 ± 11 | ||||
| Venule (n = 294) | 371 ± 15 | 406 ± 22 | 18 ± 2 | 49 ± 6* | 0 | 6.2 ± 0.3* | 0.39 ± 0.08 | 0.58 ± 0.07‡ | 37 ± 8 | 542 ± 31 | ||||
| Veins (n = 228) | 209 ± 11 | 271 ± 31 | 17 ± 3 | 32 ± 5* | 0 | 2.8 ± 0.9* | 0.37 ± 0.05 | 0.53 ± 0.05‡ | 49 ± 1 | 288 ± 29 | ||||