TABLE 2.
The number of gaps induced in confluent HUVEC monolayers under different experimental conditions
The values reported represent the number of gaps between neighboring ECs counted on 200 mm2 of monolayer surface. 5-9 different slides were used for every experimental condition. The results are means ± S.D.
| Experimental conditions | 0 min | 1 min | 5 min | 15 min | 30 min |
|---|---|---|---|---|---|
| Undisturbed | 25 ± 2 | 25 ± 4 | 24 ± 3 | 23 ± 4 | 26 ± 5 |
| PAF-treated | 25 ± 3 | 52 ± 6 | 144 ± 15 | 198 ± 19 | 312 ± 21 |
| BN-52021 + PAF | 23 ± 4 | 26 ± 5 | 25 ± 3 | 24 ± 4 | 26 ± 2 |
| siRNA Tiam1 | 26 ± 5 | 29 ± 4 | 28 ± 7 | 32 ± 6 | 31 ± 3 |
| siRNA Tiam1 + PAF | 26 ± 2 | 41 ± 4 | 81 ± 7 | 87 ± 6 | 92 ± 5 |