TABLE 2.
Antiviral activity of C34N126K peptides against C34-resistant gp41 recombinant viruses
Anti-HIV activity was determined by the MAGI assay. The data shown are the mean values and S.D. that were obtained from the results of at least three independent experiments. Shown in parentheses are the -fold increases in resistance (increase in EC50 value) calculated by comparison to a reference virus. The increase of >10-fold is indicated in bold.
|
EC50
|
|||
|---|---|---|---|
| HIV-1WTa | HIV-1ΔV4/I37K/N126K/L204Ib | ||
| nm | |||
| C34 | 1.6 ± 0.35 | 114 ± 29 (71) | |
| C34N126K | 0.95 ± 0.22 (0.6) | 1.1 ± 0.5 (0.7) | |
To improve the replication kinetics, the D36G mutation, observed in majority of HIV-1 strains, was introduced into the NL4-3 background used in this study (reference virus).
C34-resistant HIV-1 was constructed with the reference virus as described (13). ΔV4 indicates 5 amino acids deletion (FNSTW) in the V4 region of gp120.