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. 2009 Feb 20;284(8):4889–4896. doi: 10.1074/jbc.M805681200

FIGURE 1.

FIGURE 1.

Targeted gene replacement of adult mouse β globin genes with a human γ to β0 globin switching cassette. A, schematic demonstration of gene replacement in the mouse β globin locus tagged with human γβ globin and hprt genes with γβ0 globin and hyg genes. B, schematic demonstration of the wild-type mouse β globin locus and γβ0 KI allele after marker gene removal with labeled restriction endonuclease sites that confirm correct homologous recombination. The 5′ probe anneals to a 9.2-kb XbaI fragment from the wild-type allele and a 6.9-kb XbaI fragment from the human γβ0 globin KI allele. The 3′-probe derived from part of the βmin globin gene, anneals to a 14.8-kb EcoRI fragment from the βmin globin gene, a 7.3-kb fragment from the βmaj globin gene, and a 10.3-kb fragment from the γβ0 globin KI allele. C, Southern blot confirmation of mouse liver DNA of correct 5′ and 3′ homologous recombination. Lane 1, wild type control; lane 2, heterozygous γβ0 globin KI; lane 3, homozygous γβ0 globin KI.