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Example data for hydrolysis of acyl-CoA substrates. A, deacylation of acetyl-CoA substrate by RifR under steady-state conditions. The increase of the CoA fraction during the reactions of 250 μm Acetyl-CoA with 25 μm WT RifR (•), with 25 μm S94A RifR (♦), and without RifR (▪). B, Michaelis-Menten plot for deacylation of acetyl-CoA (250–1000 μm).
