TABLE 2.

Thermodynamic characterization of the interaction between LARG-RH or LARG-RDP and Gα13 at equilibrium state and in transition states using Biacore T100

Interaction phase	Thermodynamic parameter	RH	RDP
Equilibrium	ΔG° [kJ mol–1]	–43 ± 0.019	–47 ± 0.2
	ΔH° [kJ mol–1]	–85 ± 0.8	–23 ± 8.6
	ΔS° [kJ mol–1 K–1]	–0.14 ± 0.0027	0.079 ± 0.029
	TΔS° [kJ mol–1]	–43 ± 0.82	24 ± 8.8
	ΔCp° [kJ mol–1 K–1]	–5 ± 0.056	–3.8 ± 0.61
Association	ΔG°† [kJ mol–1]	38 ± 0.19	36 ± 0.11
	ΔH°‡ [kJ mol–1]	35 ± 4	61 ± 2.2
	ΔS°‡ [kJ mol–1 K–1]	–0.01 ± 0.014	0.084 ± 0.0077
	TΔS°‡ [kJ mol–1]	–3 ± 4.2	25 ± 2.3
Dissociation	ΔG°‡ [kJ mol–1]	82 ± 0.33	83 ± 0.24
	ΔH°‡ [kJ mol–1]	50 ± 6.8	32 ± 4.6
	ΔS°‡ [kJ mol–1 K–1]	–0.11 ± 0.024	–0.17 ± 0.016
	TΔS°‡ [kJ mol–1]	–32 ± 7.2	–52 ± 4.8

Thermodynamic parameters of the interactions were derived from the van't Hoff plots and Eyring plots in Fig. 4 and supplemental Fig. S5