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. 2009 Feb 20;284(8):5097–5106. doi: 10.1074/jbc.M807414200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — 13-week-old RLC phosphorylation in TG-RLC(P-) and NTG hearts. A, representative sections of narrow-range (pI 4.5–5.0) two-dimensional PAGE gels showing two phosphorylated (P2 and P1) and one unphosphorylated spot (U) of RLC in NTG and TG-RLC(P-). The gels were stained with the phospho-specific ProQ Diamond stain (top panels) and for total protein with Sypro Ruby (bottom panels), thus clearly illustrating the significant decrease in phosphorylation in TG-RLC(P-). B, percentage of RLC distribution quantified in a spot-specific manner demonstrating the significant decrease in phospho-spots P2 and P1 and increase in unphosphorylated spot U in TG-RLC(P-). The asterisk denotes statistical significance (p < 0.05; n = 3).