FIGURE 4.

PGC-1α enhances high glucose activation and GlcNAcylation of FoxO1. A, overexpression of PGC-1α in Fao hepatoma cells increases O-GlcNAc levels on co-expressed FLAG-FoxO1. The bar graph represents densitometry of relative anti-O-GlcNAc immunoreactivity from three experiments. Co-IP, co-immunoprecipitations; IB, immunoblot. B, inputs from immunoprecipitations were subjected to SDS-PAGE and blotted for O-GlcNAc, PGC-1α, OGT, and tubulin. Total O-GlcNAc levels are unchanged by overexpression of PGC-1α.