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. 2009 Feb 26;4(2):e4595. doi: 10.1371/journal.pone.0004595

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Gibbs et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) CAD, (B) LAN1 and (C) PC12 cells were heat shocked for 30 min at 42°C and allowed to recover for indicated times. (-) no heat shock. (D) CAD cells were differentiated by 36 hours serum starvation. 20 µg of protein was resolved by SDS-PAGE. Hsp70, Hsp25, Hsp27, Hsp40, Hsp90, CSPα and actin were detected by Western analysis. Data are representative of 4 separate experiments.