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. 2009 Feb 26;4(2):e4595. doi: 10.1371/journal.pone.0004595

Figure 8. Western analysis showing the association of chaperones with CSPα1-112 and CSPα1-198 before and after geldanamycin (2 µM) treatment.

Figure 8

Fusion proteins of (A) CSPα1-112 and (B) CSPα1-198 were immobilized in sepharose and incubated in the presence of 110 µg of control or geldanamycin-treated CAD cells in the presence or absence of 2 mM ATP or GDP. The beads were washed and bound proteins were eluted in sample buffer, fractionated by SDS-PAGE. Hsc70, Hsp40, and Hsp90 were detected by Western analysis.