TABLE 1.

Substrate specificities of purified Agl1_His and Agl2_His from B. breve UCC2003

Substrate	Linkage	Specific enzyme activity (μmol min−1 mg−1)a
		Agl1His	Agl2His
Disaccharides
Trehalose	O-α-d-glucosyl-(1→1)-α-d-glucose	2.0 ± 0.01	1.6 ± 0.8
Trehalulose	O-α-d-glucosyl-(1→1)-β-d-fructose	10.0 ± 0.1	11.7 ± 0.05
Sucrose	O-α-d-glucosyl-(1→2)-β-d-fructose	0.0	0.0
Turanose	O-α-d-glucosyl-(1→3)-d-fructose	0.5 ± 0.1	1.6 ± 0.7
Nigerose	O-α-d-glucosyl-(1→3)-d-glucose	0.4 ± 0.2	0.7 ± 0.2
Maltulose	O-α-d-glucosyl-(1→4)-d-fructose	0.4 ± 0.1	1.1 ± 0.63
Maltose	O-α-d-glucosyl-(1→4)-d-glucose	0.0	0.0
Cellobiose	O-β-d-glucosyl-(1→4)-d-glucose	0.0	0.0
Lactose	O-β-d-galactosyl-(1→4)-α-d-glucose	0.0	0.0
Leucrose	O-α-d-glucosyl-(1→5)-d-fructose	0.0	0.0
Palatinose	O-α-d-glucosyl-(1→6)-d-fructose	3.2 ± 0.2	4.1 ± 0.11
Isomaltose	O-α-d-glucosyl-(1→6)-d-glucose	14.1 ± 0.13	20.4 ± 0.01
Gentiobiose	O-β-d-glucosyl-(1→6)-d-glucose	0.0	0.0
Melibiose	O-α-d-galactosyl-(1→6)-d-glucose	0.0	0.0
Trisaccharides
Melezitose	O-α-d-glucosyl-(1→3)-β-d-fructosyl-(2→1)-d-glucose	0.0	0.0
Maltotriose	O-α-d-glucosyl-(1→4)-α-d-glucosyl-(1→4)-d-glucose	0.0	0.0
Raffinose	O-α-d-galactosyl-(1→6)-α-d-glucosyl-(1→2)-β-d-fructose	0.0	0.0
Panose	O-α-d-glucosyl-(1→6)-α-d-glucosyl-(1→4)-d-glucose	23.0 ± 0.21	19.3 ± 0.1
Isomaltotriose	O-α-d-glucosyl-(1→6)-α-d-glucosyl-(1→6)-d-glucose	8.6 ± 0.12	7.0 ± 0.04
Other substrates
Stachyose	β-d-fructosyl-O-α-d-galactosyl-(1→6)-O-α-d-galactosyl-(1→6)-α-d-glucose	0.0	0.0
Glycogen	(α-d-glucosyl-(1→4)-d-glucose)n, (α-d-glucosyl-(1→6)-d-glucose)n	0.0	0.0
Maltodextrins	(α-d-glucosyl-(1→4)-d-glucose)n	0.0	0.0

^aActivity was determined with 1 μg of pure enzyme and 10 mM of each substrate at 30°C for 5 min.