FIG. 1.

Vector pMP201 and derivatives. (A) Map of the pMP201 vector with polylinkers XbaI-PvuI and HindIII-EcoRI. neoA, aminoglycoside resistance gene with the promoter from the aacC4 gene; codA(s), synthetic version of the cytosine deaminase gene; ori, origin of replication for E. coli. The positions of oriT (origin of conjugative transfer), present in pMP301 and pMP302, and bla (ampicillin resistance gene), present in pMP302, are also shown. Note that the PvuI site in the polylinker is not unique in derivatives containing the bla gene. (B) DNA sequences of polylinkers in pMP201 and derivatives (coordinates in both panels are from the pMP201 sequence, and those in panel B correspond to the first boldface base pair for each restriction enzyme). Boldface letters and dashes indicate the restriction sites and the respective cleavage sites.