FIG. 3.

Production of p-hydroxystyrene by P. putida S12 427Δfcs pJNTpalpdc during a two-phase water-1-decanol nitrogen-limited fed-batch fermentation. The total concentration of p-hydroxystyrene (phs tot [⧫]; left y axis) calculated as described in the Material and Methods section, concentration of p-hydroxystyrene in the 1-decanol phase (phs dec [□]; right y axis), and the concentration of p-hydroxystyrene in the aqueous phase (phs aq [▴]; left y axis) are shown. The filled arrow indicated the time that the feed was started, and the open arrow indicates the time that 1-decanol was added. The data presented are from a single representative experiment.