TABLE 2.
Oligonucleotide primers used in this study
| Primer no. | Sequence (5′→3′)a | Description |
|---|---|---|
| 1 | GCGGCGGCCGCATGAAAAAGCGTATCGGTATTGTTG | Start of smo; forward primer |
| 2 | GCGTCTAGATCAATCAGCTCGCCATGCCCTG | Position 569-590 bp in smo; reverse primer |
| 3 | GCGTCTAGAGAAGTTCTCGCCCACACCAAG | Position 661-681 bp in smo, forward primer |
| 4 | GCGGGATCCTCAGGCCGCGATAGTCGGTGC | End of smo; reverse primer |
| 5 | GCGTCTAGAATGAGCCATATTCAACGGGAAACG | Start of Km resistance marker from pTnMod-KmO (6); forward primer |
| 6 | GCGTCTAGATTAGAAAAACTCATCGAGCATCAAATG | End of Km resistance marker from pTnMod-KmO (6); reverse primer |
| 7 | GCGGCGGCCGCGACATAAGGAAGGTAATTCTAATGAC | Start of pdc from L. plantarum; forward primer |
| 8 | GCGGCTAGCTTACTTATTTAAACGATGGTAGTTTTG | End of pdc from L. plantarum; reverse primer |
a
Restriction sites are underlined.