FIG. 1.

Chromosomal arrangement of the region containing the traHIJKCLMN genes in Y. ruckeri 150R. Arrows indicate the direction of the transcription. The organization of the transcriptional fusion between traI and the promoterless genes cat and lacZY in Y. ruckeri 150RiviXII is shown underneath, and the putative promoter (P) selected by IVET is indicated. blaseq and catseq2 oligonucleotides were used to sequence the adjacent fragments to the pIVET8 integration site. E, EcoRI sites; cat, chloramphenicol acetyltransferase gene (promoterless); lacZY, genes for lactose fermentation (promoterless); bla, ampicillin resistance gene.