TABLE 3.
Sample type and ID or cloneb | RT | % GC | Length (bp) | Closest relative | % Identity |
---|---|---|---|---|---|
FS | |||||
F3-2 | 2.58 | 227 | Uncultured euryarchaeote gene | 99 | |
Fcm12 | 12.60 | 496 | Methanosarcina siciliae | 98 | |
Fcm15 | 13.77 | 478 | Clostridium sp. | 98 | |
F3-4 | 14.01 | 369 | Uncultured euryarchaeote gene | 100 | |
Fa4 | 14.25 | 227 | Uncultured euryarchaeote gene | 99 | |
Fa6 | 14.25 | 227 | Uncultured euryarchaeote gene | 99 | |
F3-8 | 14.56 | 496 | No significant results | ||
F17m13 | 15.02 | 421 | Uncultured bacterium clone M55_D21_H_B_A08 | 97 | |
F3-2-13 | 16.95 | 751 | Uncultured euryarchaeote isolate DGGE band 672729-MODIFIZIERT | 93 | |
F3-2-14 | 16.95 | 488 | M. thermoformicicum (SF-4) | 88 | |
FA1 | 17.27 | 369 | Uncultured euryarchaeote gene | 96 | |
FA3 | 17.27 | 369 | Uncultured euryarchaeote gene | 100 | |
F23m | 17.38 | 372 | Uncultured euryarchaeote gene | 99 | |
CT | |||||
KA002 | 4.48 | 55.3 | 474 | Uncultured Flavobacterium sp. clone Y17 | 98 |
KA030 | 15.26 | 55.9 | 478 | Uncultured organism clone ctg_NISA060 | 98 |
KA039 | 14.35 | 58.9 | 474 | Uncultured bacterium clone PC-PA9-4 | 98 |
KA061 | 11.80 | 54.6 | 478 | Uncultured bacterium clone l_E07 | 97 |
KB010 | 10.95 | 493 | Uncultured archaeon gene | 97 | |
KB024 | 12.29 | 56.1 | 483 | Uncultured archaeon gene | 98 |
KB029 | 15.07 | 56.3 | 478 | Alphaproteobacterium JL1032 | 98 |
KB051 | 15.97 | 57.2 | 479 | Bacillus sp. enrichment culture clone 866 ECW 19 | 91 |
KB073 | 11.03 | 57.5 | 494 | Uncultured archaeon gene | 100 |
KB104 | 11.77 | 56.7 | 494 | Methanoculleus thermophilus strain JB-1 16S | 99 |
KB126 | 16.76 | 53.3 | 483 | Streptomyces marokkonensis strain 174443 | 99 |
KB165 | 19.22 | 57.3 | 496 | Uncultured archaeon gene | 98 |
KC009 | 12.91 | 53.9 | 498 | Uncultured archaeon gene | 96 |
KC035 | 19.25 | 60.0 | 493 | Uncultured bacterium clone 101-114 | 98 |
KC090 | 12.46 | 57.3 | 494 | Uncultured archaeon gene | 99 |
KC128 | 2.52 | 55.6 | 444 | Uncultured bacterium clone EPR3967-O2-Bc72 | 94 |
KE033 | 3.00 | 60.0 | 493 | Uncultured bacterium clone 101-114 | 98 |
KE169 | 19.22 | 60.7 | 476 | Uncultured bacterium clone C6B | 96 |
SL | |||||
B008 | 16.10 | 56.7 | 499 | Uncultured planctomycete clone Amb_16S_1421 | 96 |
B009 | 13.76 | 57.2 | 493 | Uncultured crenarchaeote 03_12a | 99 |
B014 | 13.44 | 55.8 | 493 | Uncultured crenarchaeote clone OdenE-150iia | 99 |
B018 | 14.17 | 56.1 | 476 | Uncultured soil bacterium clone 169-2 | 98 |
B024 | 17.00 | 55.1 | 477 | Uncultured proteobacterium clone Elev_16S_1449 | 98 |
B032 | 17.64 | 57.5 | 475 | Unidentified bacterium clone 61_H_RHIZO_H1_T7s | 98 |
B043 | 29.50 | 60.2 | 455 | Uncultured bacterium gene | 96 |
B046 | 18.91 | 56.0 | 479 | Uncultured bacterium clone NR.1.037 | 97 |
B103 | 15.64 | 57.2 | 479 | Uncultured bacterium gene | 99 |
B104 | 16.13 | 55.6 | 478 | Uncultured bacterium clone FCPT661 | 99 |
B105 | 16.89 | 57.1 | 476 | Acidobacteria bacterium Ellin7137 | 99 |
B111 | 13.26 | 56.2 | 495 | Uncultured archaeon clone COSAS-F3 | 99 |
B119 | 17.27 | 56.7 | 476 | Uncultured Verrucomicrobia bacterium clone 172 | 99 |
B121 | 13.42 | 56.0 | 480 | Uncultured bacterium clone C55_D6_H_B_C12 | 93 |
B123 | 18.19 | 59.3 | 492 | Uncultured euryarchaeote 03_27e | 99 |
B127 | 13.74 | 57.6 | 496 | Uncultured crenarchaeote 03_02a | 99 |
B133 | 13.14 | 56.2 | 495 | Uncultured crenarchaeote 03_06c | 100 |
B143 | 17.74 | 56.3 | 476 | Uncultured bacterium clone P1mB_062 | 100 |
B144 | 22.55 | 59.3 | 474 | Uncultured proteobacterium clone Amb_16S_520 | 100 |
B150 | 12.88 | 57.7 | 494 | Uncultured archaeon gene | 99 |
B151 | 16.20 | 55.9 | 483 | Uncultured bacterium clone BacB_007 | 99 |
B158 | 16.97 | 57.4 | 476 | Uncultured alphaproteobacterium clone KF002 | 97 |
B161 | 20.63 | 60.1 | 494 | Uncultured crenarchaeote 12_30a | 99 |
B162 | 18.95 | 56.8 | 477 | Uncultured bacterium clone BacC-u_010 | 95 |
B168 | 17.38 | 57.1 | 476 | Uncultured alphaproteobacterium clone FI-2M_B06 | 99 |
B169 | 12.95 | 56.2 | 495 | Uncultured crenarchaeote clone OdenB-100iib | 99 |
B171 | 11.13 | 54.6 | 476 | Uncultured proteobacterium clone Amb | 99 |
B172 | 12.74 | 57.3 | 494 | Uncultured archaeon gene | 99 |
B175 | 20.01 | 57.5 | 475 | Uncultured bacterium clone M55_D21_H_B_F10 | 99 |
B177 | 18.17 | 57.1 | 478 | Uncultured Holophaga sp. | 100 |
B178 | 17.62 | 57.5 | 477 | Uncultured bacterium clone Elev_16S _1673 | 100 |
B179 | 12.39 | 54.8 | 476 | Uncultured bacterium clone TSC176 | 99 |
B180 | 19.12 | 59.7 | 494 | Uncultured crenarchaeote 12_30a | 99 |
B190 | 17.20 | 57.4 | 477 | Uncultured bacterium clone FCPO684 | 99 |
B191 | 12.18 | 52.7 | 474 | Uncultured bacterium clone FCPS486 | 99 |
B205 | 11.45 | 55.9 | 494 | Uncultured archaeon gene | 98 |
B206 | 12.10 | 57.1 | 494 | Uncultured archaeon gene | 99 |
B207 | 13.01 | 476 | Uncultured soil bacterium clone 169-2 | 98 | |
B212 | 14.94 | 57.9 | 496 | Uncultured archaeon clone NAK1-a2 | 97 |
B217 | 13.22 | 57.5 | 494 | Uncultured archaeon clone GW70-20-11 16S | 100 |
B218 | 16.29 | 58.6 | 495 | Uncultured crenarchaeote clone E11_ARCH1 | 98 |
B220 | 17.53 | 57.6 | 479 | Uncultured alphaproteobacterium clone EB1049 | 100 |
B226 | 12.76 | 55.3 | 474 | Galbibacter mesophilus gene | 98 |
B229 | 16.28 | 57.0 | 474 | Rhizobiales bacterium Mfc52 gene | 100 |
B233 | 13.90 | 54.8 | 478 | Uncultured alphaproteobacterium clone lhap30 | 99 |
B238 | 18.39 | 57.7 | 477 | Uncultured Acidobacteria bacterium clone E05_WMSP1 | 98 |
B239 | 10.40 | 51.6 | 479 | Uncultured Bacteroidetes bacterium clone AKYG516 | 98 |
B244 | 16.86 | 57.4 | 479 | Uncultured Hyphomicrobiaceae bacterium clone Elev_16S_1015 | 100 |
B245 | 17.08 | 58.2 | 478 | Uncultured soil bacterium clone S013 | 100 |
B260 | 11.63 | 54.0 | 469 | Uncultured archaeon isolate DGGE band 672730-MODIFIZIERT | 87 |
B261 | 26.29 | 57.2 | 954 | No significant result | |
B288 | 17.61 | 56.0 | 495 | Uncultured archaeon clone COSAS-F3 16S | 100 |
A total of 208 clones were collected for CT in different time windows, and 289 were collected for SL.
FS, CT, and SL sample types are represented. The identification (ID) is given for the FS samples; the clone is specified for the CT and SL samples.