FIG. 3.
Transcriptional analysis of iaaLPsn and iaaLPsv in P. savastanoi pv. savastanoi. Gel electrophoresis (3% agarose) of RT-PCR amplicons of iaaL genes (A) and HaeIII-digested iaaL amplicons shown in panel A (B) are shown. Lane 1, P. syringae pv. tomato DC3000. Lanes 2 to 6 correspond to P. savastanoi pv. savastanoi strains. Lanes: 2, ITM 317; 3, NCPPB 3335; 4, NCPBB 639; 5, IVIA 1637-B3; 6, IVIA 1637-C1; and 7, NCPPB 64. Lane C+, positive control RT-PCR amplification reaction with total DNA of NCPPB 3335 as the template. Lane L, 50-bp DNA ladder (Promega Co.); the positions (in base pairs) of the molecular size markers are indicated on the left of the gel.