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. 2008 Dec 19;75(4):1030–1035. doi: 10.1128/AEM.01572-08

TABLE 1.

Bacterial strains used in this study

Name and straina Country (province)b iaaL hybridization
iaaLPsv size (bp)e (TAC)nf Reference or source
No.c Classificationd
P. syringae pv. tomato
    DC3000 United Kingdom 1C 3 9
P. savastanoi pv. nerii
    ITM519 Italy 2P 3 45
    LPVM Psn2 Italy 1P 3 S. Tegli
P. savastanoi pv. savastanoi
    NCPPB 1506 Italy 2C II 122 3 29
    ITM 317h Italy 2C 122 3 45
    PVFi 1 Italy 2C 122 3 18
    IVIA 2733-1a Italy 2C 122 3 35
    CFBP 1020 France 2C II 122 3 29
    CFBP 2074h Algeria 2C 122 3 29
    NCPPB 1342 United States 2C II 128 5 29
    NCPPB 1344h United States 2C II 128 5 29
    NCPPB 3335h France 2C 128 5g 29
    IVIA 1628-3h Spain (Valencia) 2C 128 5g 29
    IVIA 2743-3 Spain (Badajoz) 2C II 128 5 35
    GUMA Psv160 Spain (Jaén) 2P (25-95) III 131 6 This study
    GUMA Psv185 Spain (Málaga) 2C I 131 6 This study
    LSV C3.01 Spain (Sevilla) 2C I 131 6g 32
    IVIA 2445-4 Spain (Jaén) 1P (70)/1C II 131 6 35
    IVIA 1624-b1h Spain (Valencia) 1P (75)/1C II 131 6 29
    NCPPB 2327 Italy 1P (60)/1C II 131 6 29
    NCPPB 1479 Serbia 1P (65)/1C II 131 6 29
    NCPPB 639Th Serbia 1P (90)/1C II 131 6 16
    LSV C1.01 Spain (Sevilla) 2C II 137 8 32
    LSV B15.00 Spain (Sevilla) 2C I 137 8 32
    IVIA 1649-1h Spain (Castellón) 2C I 137 8g 29
    LSV C2.01 Spain (Sevilla) 2C I 140 9 31
    IVIA 1657-a2 Spain (Alicante) 2C I 140 9 29
    IVIA 1637-B3h Spain (Alicante) 2C III 143 10g 29
    IVIA 1629-1a Spain (Valencia) 2C I 149 12 29
    IVIA 1637-C1 Spain (Alicante) 2C I 151 13g 29
    IVIA 1651-c15 Spain (Alicante) 2C I 155 14g 29
    NCPPB 64h Portugal 2C I 155 14 29
    IVIA 1657-b8h Spain (Valencia) 2C I 158 15g 29
a

ATCC, American Type Culture Collection, Manassas, VA; CFBP, Collection Francaise des Bacteries Phytopathogenes, Angers, France; IVIA, Instituto Valenciano de Investigaciones Agrarias Collection, Valencia, Spain; LPVM, Laboratorio di Patologia Vegetale Molecolare Collection, Firenze, Italy; LSV, Laboratorio de Sanidad Vegetal Collection, Sevilla, Spain; NCPPB, National Collection of Plant Pathogenic Bacteria, York, United Kingdom; GUMA, Área de Génetica, Universidad de Málaga Collection, Málaga, Spain.

b

The province of isolation is indicated only for Spanish strains.

c

Number of chromosomal (C) and plasmid (P)-borne copies of iaaL. The size (in kilobases) of iaaL-hybridizing plasmids is indicated in parentheses.

d

Classification groups for iaaL hybridization patterns of P. savastanoi pv. savastanoi strains exhibiting a common BglII hybridization band of approximately 8 kb. I, banding pattern exhibits a second hybridization band at 20 kb (Fig. 1, lanes 8 and 10 to 14); II, banding pattern exhibits an additional hybridization band larger than 9.4 kb and smaller than 20 kb (Fig. 1, lanes 6, 7, 16, and 17); and III, banding pattern exhibits an additional hybridization band larger than 23 kb (Fig. 1, lane 9).

e

Size of PCR-amplified iaaLPsv fragments calculated by capillary electrophoresis.

f

Estimated number of TAC repeats in the iaaL gene. For P. savastanoi pv. savastanoi strains, the number of TAC repeats refers to the iaaLPsv paralog.

g

Strain with both iaaL paralogs sequenced. Accession numbers of iaaLPsv fragments are as follows: NCPPB 3335 (EU616802), IVIA 1628-3 (EU616795), LSV C301 (EU616796), IVIA1649-1 (EU616800), IVIA 1637-B3 (EU616799), IVIA 1637-C1 (EU616798), IVIA 1651-c15 (EU616801), and IVIA 1657-b8 (EU616797).

h

Strain with partially sequenced gyrB and rpoD genes. Nucleotide sequences exhibited 100% identity with those of P. savastanoi pv. savastanoi strain NCPPB 639 (accession numbers AB039514 and AB039469 for rpoD and gyrB, respectively).