FIG. 4.

Phylogenetic analysis of nifH genes from isolates nodulating L. tenuis. Aligned sequences of nifH genes that were 500 bp long (positions 317 to 815 of the nifH nucleotide sequence from M. loti MAFF303099) were used. The tree was constructed by using the neighbor-joining method. The percentages of replicate trees in which the isolates and reference strains clustered together in the bootstrap test (1,000 replicates) are indicated at branch points (only values greater than 50% are shown). The tree is drawn to scale, and the units for the branch lengths are the same as the units for the phylogenetic distances used to infer the phylogenetic tree.