TABLE 2.
Metabolism of benzoate and fluorobenzoate isomers by pure cultures of S. aciditrophicus grown with crotonatea
| Substrate(s) | Maximum OD600b | Crotonate used (mM) | Cosubstrate used (mM) | Acetate formed (mM) | CHAC formed (mM) | Fluoride formed (mM) | Benzoate formed (mM) | Final H2 (Pa) |
|---|---|---|---|---|---|---|---|---|
| Crotonate | 0.24 ± 0.01 | 7.1 ± 0.2 | NA | 10.4 ± 2.1 | 1.16 ± 0.08 | NA | 0.04 ± 0.01 | 9.4 ± 1.2 |
| Crotonate + benzoate | 0.25 ± 0.01 | 7.7 ± 0.4 | 1.79 ± 0.12 | 15.1 ± 0.9 | 1.67 ± 0.26 | NA | NA | 8.9 ± 2.3 |
| Crotonate + 2-fluorobenzoate | 0.09 ± 0.01 | 2.2 ± 0.5 | 0.06 ± 0.03 | 2.2 ± 2.0 | 0.17 ± 0.01 | 0.06 ± 0.01 | 0.04 ± 0.01 | 87.4 ± 7.8 |
| Crotonate + 3-fluorobenzoate | 0.08 ± 0.01 | 3.7 ± 0.6 | 0.13 ± 0.02 | 5.7 ± 0.3 | 0.53 ± 0.14 | 0.15 ± 0.02 | 0.17 ± 0.01 | 116.6 ± 13.9 |
| Crotonate + 4-fluorobenzoate | 0.25 ± 0.01 | 6.7 ± 1.0 | 0.01 ± 0.02 | 9.9 ± 1.8 | 1.3 ± 0.20 | 0.005 ± 0.14 | 0.04 ± 0.01 | 7.8 ± 0.8 |
a
Abbreviations: OD600, optical density at 600 nm; CHAC, cyclohexane carboxylate; NA, not applicable. The values are the averages ± standard deviations of triplicate cultures measured after 10 days of incubation.
b
The maximum absorbance was reached within 6 days for the cultures grown in the presence of crotonate alone or crotonate and 4-fluorobenzoate; it was reached within 10 days for the cultures grown with crotonate and benzoate, 2-fluorobenzoate, or 3-fluorobenzoate.