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Summary of ScoT mutant activities
| Substrate | ScoT | kcat (min−1) | Km (mM) | kcat/Km (M−1 s−1) |
|---|---|---|---|---|
| p-NP | Wild type | 3,567 | ||
| propionate | S90A | Inactive | Inactive | Inactive |
| S90C | 47 ± 8 | 2.09 ± 0.54 | 378 | |
| H210Y | 540 ± 79 | 1.99 ± 0.48 | 4,523 | |
| H224Y | 313 ± 41 | 3.65 ± 0.66 | 1,428 | |
| NAC | Wild type | 450 ± 67 | 34 ± 10 | 221 |
| propionate | S90A | Inactive | Inactive | Inactive |
| S90C | 24 ± 5 | 24 ± 12 | 17 | |
| H210Y | 320 ± 12 | 18 ± 2 | 303 | |
| H224Y | 150 ± 6 | 20 ± 2 | 122 |
