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. 2008 Dec 1;8(2):190–196. doi: 10.1128/EC.00201-08

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FIG. 3. — mycGAP45 associates with other members of the myosin XIV motor complex. (A) GAP45 antiserum immunoprecipitates a complex of MyoA, MLC1, GAP45, and GAP50 from [³⁵S]methionine-cysteine-labeled wild-type parasites. With parasites expressing mycGAP45, immunoprecipitation with anti-myc pulls down mycGAP45, MyoA, and MLC1, demonstrating that addition of the myc tag does not interfere with assembly of the myosin XIV motor complex. GAP50 and GAP45/mycGAP45 migrate at the same molecular weight on SDS-PAGE gels. (B) Nontransfected parasites and parasites expressing mycGAP45 were analyzed by gel filtration chromatography on Superdex 200 columns, and the fractions were analyzed by immunoblotting for the various members of the myosin XIV motor complex. The subunits of the mature complex, MyoA, GAP50, GAP45/mycGAP45, and MLC1, all coeluted in the higher-molecular-size fractions, peaking in fractions 18 and 19. The fractions containing thyroglobulin (669 kDa) and catalase (232 kDa) are indicated by arrows.