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. 2008 Dec 1;8(2):190–196. doi: 10.1128/EC.00201-08

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Copyright © 2009, American Society for Microbiology

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FIG. 5. — GAP45 phosphorylation is involved in assembly of the myosin XIV motor complex. Parasites transiently expressing mycGAP45 (wt), mycGAP45(Ser¹⁶³Ala), mycGAP45(Ser¹⁶³Glu), mycGAP45(Ser¹⁶⁷Ala), mycGAP45(Ser¹⁶⁷Glu), mycGAP45(Ser¹⁶³Ala, Ser¹⁶⁷Ala), and mycGAP45(Ser¹⁶³Glu, Ser¹⁶⁷Glu) were immunoprecipitated with a monoclonal antibody to the myc epitope. Immunoprecipitates were analyzed by SDS-PAGE and immunoblotting with monospecific rabbit antisera to MyoA, GAP50, GAP45, and MLC1.