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Ras1 palmitoylation is required for membrane localization. The localization patterns of the GFP-RAS1, GFP-ras1(C203S), GFP-ras1(C204S), GFP-ras1(C203S, C204S), and GFP-ras1(C207A) transformants were determined using confocal microscopy. Cells were imaged (×63 objective). Bar, 10 μm.
