TABLE 2.
The essential function of S. pombe tbf1 can be provided by expression of P. carinii tbf1 but not S. cerevisiae TBF1a
| Plasmid transformed | No. of viable colonies plated on minimal medium + Leu | No. of G418-resistant haploid colonies on replica plating | % of viable colonies that carry tbf1::Kanr |
|---|---|---|---|
| Vector | 580 | 0 | 0 |
| Sptbf1 | 545 | 211 | 39 |
| Pctbf1 | 129 | 64 | 50 |
| ScTBF1 | 196 | 0 | 0 |
a
Spores from the tbf1/tbf1::KanMX4 diploid transformed with the indicated plasmids were plated at approximately equivalent densities and allowed to germinate on medium selective for the presence of plasmid. The number of haploid colonies (adenine prototrophs), able to grow on medium supplemented with G418, was established by replica plating and calculated as a percentage of the total number of viable colonies.