FIG. 3.

Mass spectrometric analysis of intact sVSG221 from wild-type and TbUGGT null mutant trypanosomes treated with and without 1-deoxynojirimycin. (A) Scheme of the primary structure of wild-type VSG221 used as a reporter of protein N glycosylation in this study (top) and wild-type VSG made in the presence of 1-deoxynojirimycin (bottom). Samples of whole sVSG were analyzed by ES-MS, and the spectra were deconvolved. The sVSG samples were from wild-type cells (B), TbUGGT null mutant cells (C), wild-type cells grown in the presence of 1-deoxynojirimycin (D), and TbUGGT null mutant cells grown in the presence of 1-deoxynojirimycin (E). Rel. int., relative intensity.