TABLE 1.

N-glycans expressed by wild-type and GII null mutant trypanosomes

^aThe peak identifiers and retention times refer to the Dionex HPAEC chromatograms shown in Fig. 1C and D.

^bThe positive-ion ES-MS analyses of the individual peaks revealed the main molecular species as being [M+H]⁺, [M+2H]²⁺, or [M+2Na]²⁺ ions from which the glycan compositions were deduced.

^cThe proportions of each glycan are estimated from the areas of the peaks in Fig. 1C and D. WT, wild type. Underlined values indicate N-glycans retaining one terminal κ-Glc residue.

^dThe proposed structures are based on the known compositions, the ES-MS-MS spectra of the principal ions shown in Fig. 1A and B, and literature precedent for T. brucei N-glycans with those compositions.

^eLikely origin of the N-glycan with respect to the OST (OST1 or OST2) (39) that will have transferred its Man₅GlcNAc₂ (OST1) or Man₉GlcNAc₂ (OST2) precursor.