TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a |
|---|---|
| MEP3 | TAAATACGGTACCCAAACGATTGGCTTGAATGTC |
| MEP9 | CCATGAGTTGAGATCTCAGTATCATTGTG |
| MEP23 | AGAAATCAGATCTACACTTCAACGTAATCATAAGC |
| MEP32 | AAAGAACTGGATCCATTTTTAGCTTCTCC |
| MEP38p | CCGGCGAACAACAATGCACCTAAGC |
| MEP39p | TGGCGGTCCAGTTCACGAAAACTCTGG |
| MEP59 | CAGCATAATAGATCTTATTCACCAATTTGGG |
| MEP67p | CCGGCGAAATTCAATGCACCTAAGC |
| MEP68p | CAGCAGCAAACATCCCTTGGGCAAGACAGAAC |
| MEP69p | CAGCAGCAGCCATCCCTTGGTAAAGACAGAAC |
| MEP70p | TCACTGCTATTTTGATGGCTGGTGC |
| MEP71p | CCGGCGAAATCCAATGCACCTAAGC |
| MEP72p | TGGCGGTCCAGTTGCCGAAAACTCTGG |
| MEP73p | ACAGTAAACAACAGTCAACCAG |
| MEP74p | CCTATTGCCTATGCCACATGGGGTGG |
| MEP75p | GGCCCCACCGTTGAAACCGTACC |
| MEP76p | ACTGGTAACTCTTCCATGCGTTC |
| MEP77p | GTCCATACCATCATCAATCTGC |
| MEP78p | GTGTGGGCACTCGCTGGTGTTGGTGG |
| MEP80p | CCTATTGCCTATTGGACAGCCGGTGGAAATGG |
| MEP81 | TTGGTTTCAGATCTAGTCATCAGCATAATAGG |
| MEP82 | TTGGTTTCAAGATCTAATCAGCATAATAGG |
| MEP83 | TTGGTTTCAGGATAGATCTAAGCATAATAGGCATATTCACC |
| MEP84 | TTCAGGATCGTAGATCTAATAATAGGCATATTCACC |
| MEP85 | TTCAGGATCGTCATCAGATCTAATAGGCATATTCACC |
| MEP86 | ATCGTCATCAGCAGATCTAGGCATATTCACCAATTTGG |
| MEP87 | ATCGTCATCAGCATAGATCTAATATTCACCAATTTGGG |
| MEP91 | TCACTTCAAAGATCTAATAAGCAAATTCACCAATTTG |
| MEP94p | GGACCCACCGTTGGCACCGTACCAACC |
| MEP95 | ATTTAGATCTAATAGGCAAATTCACCAATTTG |
| MEP96 | ATTTAGATCTAATAGGCATATTCACCAATTTGATCCAAGTCGGTTCC |
| MEP97 | ATTTAGATCTAATAGGCATATTCACCAATTTGGGCTTCGTCGGTTCC |
| MEP98 | ATTTAGATCTAATAGGCATATTCACCAATTTGGGCCAAGTCAACTCCCAACATTTC |
| MEP99 | ATTTAGATCTAATAGGCATATTCACCAATTTGGGCCAAGTCGGTTCCAGCCATTTCCTCGTC |
| MEP100 | ATTTAGATCTAATAGGCATATTCACCAATTTGGGCCAAGTCGGTTCCCAATTCTTCCTCGTCTTC |
| MEP101 | ATTTAGATCTAATAGGCATATTCACCAATTTGGGCCAAGTCGGTTCCCAACATTTCTCCGTCTTCGTGGAG |
| MEP102 | ATTTAGATCTAATAGGCATATTCACCAATTTGGGCCAAGTCGGTTCCCAACATTTCCTCATTTTCGTGGAGACG |
a
Restriction sites introduced into the primers are underlined; stop codons (reverse sequence) are highlighted in bold, and mutated codons are shown in italics. Primers with the suffix “-p” are 5′ phosphorylated.