FIG. 2.

Secretion of sICAM-1, IL-8, IL-6, MCP-1, MIP-1α, and IL-1α by KB and MRC-5 cells following incubation with viable P. gingivalis W50 whole cells. Confluent KB and MRC-5 cell monolayers were incubated with P. gingivalis W50 at various BCRs (10:1. 50:1, 100:1 500:1, 1,000:1, and 10,000:1 for 90 min at 37°C. After incubation, P. gingivalis was removed, and the cell monolayers were incubated with fresh culture medium for a further 16 h at 37°C. Following incubation, the culture supernatants were collected and assayed for sICAM-1, IL-8, IL-6, MCP-1, MIP-1α, and IL-1α by ELISA. The values represent the means and standard deviations of triplicate determinations from three representative experiments. The dotted line represents basal cytokine levels as determined by unstimulated cells (control). Data were analyzed by a t test and effect size (Cohen's d) compared to the control.