FIG. 2.

(a) Growth of UPEC strain CFT073 and metal transport-defective mutant derivatives in M9-glucose medium. Symbols: ▪, CFT073 wild type; ▴, Δznu mutant (QT1434); ▾, ΔzupT mutant (QT1554); ⧫, Δznu ΔzupT mutant (QT1555); •, Δznu ΔsitABCD mutant (QT1640); ×, Δznu ΔzupT ΔsitABCD mutant (QT1634). (b) Growth rescue of the UPEC Δznu ΔzupT strain QT1555 in M9-glucose medium supplemented with 5 μM Zn²⁺ (▪), Mn²⁺ (░⃞), or Fe³⁺ (□). Growth of the strain is presented as the percent growth compared to the wild-type strain CFT073 based on the formula: [(OD₆₀₀ of the mutant in metal-enriched medium) − (OD₆₀₀ of the mutant)]/(OD₆₀₀ of the wild-type strain) × 100.