TABLE 2.
Primers used in this study
| Primer | Characteristic(s) | Sequence | Tm (°C) |
|---|---|---|---|
| CMD23 | SitA-SitD region amplification (used with CMD42) | CGCAGGGGGCACAACTGAT | 54 |
| CMD42 | CTGTGCGCTGCTGTCGGTC | 54 | |
| CMD264 | Amplification of znu::kan from JWK5831_1 (used with CMD265) | AAAACGCCACAATCCAGTTC | 54 |
| CMD265 | CGGCAGTACGGTCATAGGTT | 56 | |
| CMD637 | Contains a HindIII restriction site; amplification of znuACB region of MG1655 for cloning (used with CMD638) | GACAAGCTTGGCCAGAGTAAGAACGG | 56 |
| CMD638 | Contains a HindIII restriction site | CGAAAGCTTCGTGGAATCACTTTGGC | 56 |
| CMD715 | Construction of zupT::kan using pKD13 template plasmid (used with CMD716) | TTCCCGCCGTTTGTAGCAGCACTAAACTGAATCCCATCACTGACATTCCAATTCCGGGGATCCGTCGACC | 68 |
| CMD716 | CTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTTGTAGGCTGGAGCTGCTTCG | 68 | |
| CMD717 | Contains a HindIII restriction site; amplification of zupT operon of MG1655 for cloning (used with CMD718) | GTTAAGCTTACGATCTGCCTGAAGGTGAA | 54 |
| CMD718 | Contains a HindIII restriction site | GAAGAAGCTTATGCGCTGGACAACTTCTG | 54 |