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Infection and replication of different Brucella species within human osteoblasts. After infection at different MOIs, cells were incubated with antibiotics to kill extracellular bacteria. Cells were lysed at different times p.i. and plated on agar to determine intracellular CFU. Values are means ± standard errors of the means (SEM) for triplicate determinations from one experiment, which was repeated twice with similar results.
