FIG. 4.

Effects of cytoskeleton inhibitors on B. abortus internalization in osteoblasts. Osteoblasts were exposed to each compound during the whole infection period (2 h) with B. abortus at an MOI of 1,000. After being washed, the cells were incubated for 2 h in the presence of gentamicin and processed as indicated in the legend to Fig. 1. Data represent means ± SEM of CFU measured in triplicate in one experiment, which was repeated twice with similar results. Significant differences relative to the control infection (no inhibitor added) are indicated with asterisks (*, P < 0.05; **, P < 0.01 [ANOVA followed by Dunnett's multiple comparison test]).