FIG. 1.

M. genitalium G37 induced TLR2/6-mediated NF-κB activation. HEK293 cells, stably expressing human TLR2/6, TLR2 (A), or TLR5 (B) were transfected with an NF-κB-responsive SEAP reporter plasmid to quantify NF-κB activation in response to M. genitalium exposure. Extensively washed viable M. genitalium cells (MOI, 1 to 100) were applied to HEK293 cells, and then culture supernatants were collected 24 h postincubation for SEAP quantification. Purified recombinant S. enterica serovar Dublin FLAG and FSL-1 from M. salivarium were used as TLR specificity controls and processed in parallel on each cell type. Data shown are the means ± standard errors of the means of SEAP induction collected in two independent experiments performed in triplicate wells. Statistical comparisons were made using one-way ANOVA followed by Dunnett's post test. *, P < 0.01 versus PBS vehicle control.