FIG. 10.

Effect of Sm16(23-117)^AAP on TLR ligand-induced intracellular signaling events. Mono-Mac-6 cells were preincubated for 10 min in the absence or presence of Sm16(23-117)^AAP (20 μg/ml) as indicated. A buffer control (Co), LPS (50 ng/ml), or PG (5 μg/ml) was then added as indicated. Cells were incubated at 37°C for 0.5 h (A), 1 h (B, top), or 8 h (B, bottom) and processed for immunoblot analysis. Filters were probed with either IκB-α (A) or anti-IRAK1 (B). Anti-α-tubulin was used as a control for equal loading. The presented data are representative of at least three independent analyses.