FIG. 7.
Effect of Sm16(23-117)AAP on cytokine production in response to various TLR ligands. Human blood was preincubated for 10 min with a buffer control (open bars) or P. pastoris-expressed Sm16(23-117)AAP (20 μg/ml) (filled bars). The buffer control, LPS (50 ng/ml), poly(I:C) (20 μg/ml), PG (5 μg/ml), or Pam3CSK4 (5 μg/ml) was then added as indicated for 6 h of incubation, followed by analysis of either IL-6 (upper panel) or IL-1RA (lower panels) in the supernatants. The data plotted are representative of at least four independent analyses performed in triplicate with blood from three donors.