TABLE 3.

Results of restriction enzyme analysis, denoted by profiles A to J, and normalized HRM-curve analysis, indicating genotype and mean C% of sample replicates for 12 FAdV reference serotypes

Serotype	Restriction enzyme profile	HEX-S		Hexon A/B		Hex L1
		Genotypea	Mean C% ± SEb	Genotype	Mean C% ± SE	Genotype	Mean C% ± SE
FAdV-1	A	1	99.6 ± 0.16	1	99.5 ± 0.21	1	99.6 ± 0.18
FAdV-2	B	2	99.5 ± 0.21	2	99.1 ± 0.21	2	99.2 ± 0.35
FAdV-3	C	3	98.9 ± 0.47	3	99.1 ± 0.34	3	99.8 ± 0.07
FAdV-4	D	4	99.5 ± 0.20c	4	99.1 ± 0.30	4	99.8 ± 0.10
		10	99.3d
FAdV-5	NAe	5	99.2 ± 0.36	NAe	NAe	5	99.8 ± 0.06
FAdV-6	E	6	99.2 ± 0.02c	6	99.7 ± 0.09	6	99.9 ± 0.03
		11	98.9d
FAdV-7	F	7	99.0 ± 0.44	7	99.5 ± 0.18	7	99.1 ± 0.42
FAdV-8a	G	8a	98.5 ± 0.72	8a	99.5 ± 0.18	8a	99.5 ± 0.23
FAdV-8b	H	8b	99.8 ± 0.08	8b	98.8 ± 0.56	8b	99.1 ± 0.19
FAdV-9	I	9	99.5 ± 0.36	9	99.4 ± 0.24	9	99.3 ± 0.33
FAdV-10	J	10	99.4 ± 0.70c	10	99.6 ± 0.20	10	99.8 ± 0.05
		4	99.4d
FAdV-11	B	11	99.6 ± 0.16	11	99.8 ± 0.07	11	99.5 ± 0.24

^aGenotypes were defined by selecting a representative sample from each serotype.

^bThe mean C% of three replicate samples per serotype is shown (unless otherwise indicated), plus or minus the SE.

^cThe mean C% of two replicate samples per serotype is shown, plus or minus the SE.

^dOne replicate sample of the serotype was assigned to a representative genotype of a different serotype; therefore, the mean C% and SE were not calculated.

^ePrimers Hexon A and Hexon B did not produce a PCR amplicon for FAdV-5; therefore, restriction enzyme analysis and HRM-curve analysis were not performed on these samples. NA, not available.